- Robert Lowery
- Batman / Bruce Wayne
- Johnny Duncan
- Robin / Dick Grayson (as John Duncan)
- Jane Adams
- Vicki Vale
- Lyle Talbot
- Commissioner Jim Gordon
- Ralph Graves
- Winslow Harrison [Chs. 7-8]
- Don C. Harvey
- Nolan - Henchman [Chs. 1-6]
- William Fawcett
- Prof. Hammil
- Leonard Penn
- Carter - Hammil's Valet
- Rick Vallin
- Barry Brown
- Michael Whalen
- Dunne - Private Investigator [Chs. 2, 9, 12]
- Greg McClure
- Evans - Henchman [Chs. 1-6]
- House Peters Jr.
- Earl - Henchman [Chs. 7-15]
- Jim Diehl
- Jason - Henchman [Chs. 3-15]
- Rusty Wescoatt
- Ives - Henchman [Chs. 3-13]
- Phil Arnold
- Doctor [Ch. 4]
- Marshall Bradford
- Wesley Morton [Chs. 3-4]
- Jack Chefe
- Plant Guard in Hallway [Chs. 13-14]
- George Cisar
- Policeman in Alley [Ch. 1]
- Richard Cramer
- Newspaper Office Worker [Ch. 7]
- James Craven
- Norwood [Chs. 1, 3, 12-14]
- John Doucette
- Henchman [Ch. 2, 3, 6, 10-12, 15]
- Kenne Duncan
- Gabe
- Frank Ellis
- Policeman Assigned to Set Up Jason's Escape [Ch. 10]
- Frank Hagney
- Plant Guard [Ch. 14]
- John Hart
- John Hench / Mechanic [Ch. 2] (uncredited)
- Myron Healey
- Walker - Policeman [Ch. 7]
- Knox Manning
- Narrator
- Frank O'Connor
- Plant Gate Guard [Chs. 13-14]
- George Offerman Jr.
- Jimmy Vale - Henchman [Chs. 1-2, 5, 8-11]
- Eddie Parker
- Holt [Chs. 1, 5-8, 12, 15]
- Allan Ray
- Mac Lacey [Ch. 5]
- Lee Roberts
- Neal - Henchman [Chs. 7-15]
- George Robotham
- Car 4 Henchman-Driver [Chs. 2-6, 11-12]
- Guy Teague
- Factory Guard [Ch. 1] / Aircraft Maintenance Man [Ch. 2] (uncredited)
- Emmett Vogan
- Mr. Williams [Chs. 1, 3, 12-14]
- Wally West
- Pursuit Cop [Ch. 15]
- Eric Wilton
- Alfred Beagle
