- Claudia Cardinale
- Jill McBain
- Henry Fonda
- Frank
- Jason Robards
- Manuel 'Cheyenne' Gutiérrez
- Charles Bronson
- Harmonica
- Gabriele Ferzetti
- Morton
- Paolo Stoppa
- Sam
- Woody Strode
- Stony
- Jack Elam
- Snaky
- Keenan Wynn
- Sheriff
- Frank Wolff
- Brett McBain
- Lionel Stander
- Barman
- Livio Andronico
- Bit part
- Salvatore Basile
- Member of Cheyenne's Gang
- Aldo Berti
- Member of Frank's Gang Playing Poker
- Regina Elena Bisio
- Old Woman in the Tavern
- Joseph Bradley
- Train Station Master
- Frank Braña
- Member of Frank's Gang Smoking Pipe at Auction
- Marilù Carteny
- Mourner at Brett McBain's Funeral
- Saturno Cerra
- Member of Frank's Gang on Train
- Luigi Ciavarro
- Older Sheriff's Deputy
- Spartaco Conversi
- Member of Frank's Gang Shot Through Boot
- Bruno Corazzari
- 3rd Member of Cheyenne's Gang
- Bill Crawley
- Railway Worker
- Paolo Figlia
- Member of Frank's Gang
- John Frederick
- Jim - Member of Frank's Gang
- Don Galloway
- Member of Frank's Gang in Flashback
- Michael Harvey
- Frank's Lieutenant
- Robert Hossein
- Member of Frank's Gang in Flashback
- Stefano Imparato
- Patrick McBain
- Francesca Leone
- Girl at Flagstone Station
- Raffaella Leone
- Girl at Flagstone Station
- Frank Leslie
- Member of Frank's Gang in Flashback
- Luigi Magnani
- Unconfirmed Role
- Claudio Mancini
- Harmonica's Brother
- Dino Mele
- Harmonica as a Boy
- Antonio Molino Rojo
- Member of Frank's Gang at Auction
- Enrico Morsella
- Member of Frank's Gang in Flashback
- Umberto Morsella
- Member of Frank's Gang in Flashback
- Al Mulock
- Knuckles - Member of Frank's Gang
- Ricardo Palacios
- Morton's Train Conductor
- Tullio Palmieri
- Flagstone Carpenter
- Antonio Palombi
- Cattle Corner Station Agent
- Renato Pinciroli
- First Bidder at Auction
- Lorenzo Robledo
- 2nd Member of Cheyenne's Gang
- Sandra Salvatori
- Mourner at Brett McBain's Funeral
- Aldo Sambrell
- Cheyenne's Lieutenant
- Conrado San Martín
- Vecino
- Enzo Santaniello
- Timmy McBain
- Simonetta Santaniello
- Maureen McBain
- Claudio Scarchilli
- Member of Frank's Gang
- Giovanni Ivan Scratuglia
- Sheriff's Deputy
- Robert Spafford
- Construction Yard Owner
- Benito Stefanelli
- Frank's Lieutenant
- Simonetta Stefanelli
- Maureen McBain
- Luana Strode
- Indian Woman at Cattle Corner Station
- Fabio Testi
- Member of Frank's Gang with Black Hat at Auction
- Dino Zamboni
- Man in the Bar
- Marco Zuanelli
- Wobbles
