- Marlene Dietrich
- Frenchy
- James Stewart
- Tom Destry Jr.
- Mischa Auer
- Boris Callahan
- Charles Winninger
- Washington Dimsdale
- Brian Donlevy
- Kent
- Allen Jenkins
- Gyp Watson
- Warren Hymer
- Bugs Watson
- Irene Hervey
- Janice Tyndall
- Una Merkel
- Lily Belle
- Billy Gilbert
- Loupgerou
- Samuel S. Hinds
- Judge Slade
- Jack Carson
- Jack Tyndall
- Tom Fadden
- Lem Claggett
- Virginia Brissac
- Sophie Claggett
- Edmund MacDonald
- Rockwell
- Lillian Yarbo
- Clara
- Joe King
- Sheriff Keogh
- Dickie Jones
- Claggett Boy
- Ann E. Todd
- Claggett Girl
- Richard Alexander
- Cowboy
- C.E. Anderson
- Townsman
- Silver Tip Baker
- Barfly
- John Barton
- Townsman
- Chief John Big Tree
- Indian in Saloon
- Billy Bletcher
- Pianist
- Rudy Bowman
- Townsman
- Ed Brady
- Barfly
- Loren Brown
- Juggler
- Buck Bucko
- Barfly
- Roy Bucko
- Barfly
- Bob Card
- Barfly
- George Chesebro
- Barfly
- Noble 'Kid' Chissell
- Townsman
- Dora Clement
- Woman
- Bill Cody Jr.
- Townsboy Telling Wash of Destry's Arrival
- Spade Cooley
- Fiddle Player
- Harry Cording
- Creepy - Lends Tom Guns
- Carmen D'Antonio
- Dancer
- Harold DeGarro
- Juggler
- Tex Driscoll
- Barfly
- Florence Dudley
- Small Role
- Ruth Eddings
- Friend of Lilly Belle
- O.K. Ford
- Barfly
- Sam Garrett
- Rider / Roper (uncredited)
- Slim Gaut
- Townsman
- William Gillis
- Barfly
- Jack Gordon
- Barfly
- Lloyd Ingraham
- Express Agent with Box of Rabbits
- Marjorie Kane
- Saloon Floozie
- Paul King
- Waiter
- Jackie Krenk
- Child
- Harley Luse
- Accordion Player
- Johnny Luther
- Barfly
- Cactus Mack
- Musician
- Kermit Maynard
- Cowhand
- Frank McCarroll
- Barfly
- Bud McClure
- Stage Driver
- Merrill McCormick
- Townsman
- Philo McCullough
- Bartender
- Robert McKenzie
- Doctor
- Robert Milasch
- Townsman
- Charles Murphy
- Townsman
- Tex Parker
- Townsman
- Jim Reeves
- Townsman
- Carl Sepulveda
- Greets Stage
- Mary Shannon
- Woman on Street
- Rudy Sooter
- Bass Player
- Betta St. John
- Singing Girl in Wagon
- William Steele
- Cowboy
- Leo Sulky
- Bartender
- Al Taylor
- Townsman
- Eleanor Taylor
- Child singing in wagon
- Harry Tenbrook
- Stage Shotgun Rider
- Jack Tornek
- Barfly
- Minerva Urecal
- Mrs. DeWitt
- Hank West
- Musician
- Dan White
- Barfly
- Blackie Whiteford
- Juror
- Alex Woloshin
- Assistant Bartender
- Duke York
- Townsman
