- Lionel Atwill
- Dr. Jerry Xavier
- Fay Wray
- Joanne Xavier
- Lee Tracy
- Lee Taylor
- Preston Foster
- Dr. Wells
- John Wray
- Dr. Haines
- Harry Beresford
- Dr. Duke
- Arthur Edmund Carewe
- Dr. Rowitz
- Leila Bennett
- Mamie
- Robert Warwick
- Police Commissioner Stevens
- George Rosener
- Otto
- Willard Robertson
- Detective O'Halloran
- Thomas E. Jackson
- Daily World Editor
- Harry Holman
- Mike - Waterfront Policeman
- Mae Busch
- Cathouse Madam
- Tom Dugan
- Sheriff
- Louise Beavers
- Louise the Cathouse Maid
- Raoul Freeman
- Morgue Detective
- Selmer Jackson
- Willard Keefe - Daily World Night Editor
- Charles McMurphy
- Detective at Headquarters
- Ky Robinson
- Morgue Detective