- Tom Hanks
- Self - Narrator
- Regen Wilson
- Peter Taltavul
- Billy Campbell
- Abraham Lincoln
- Jesse Johnson
- John Wilkes Booth
- Geraldine Hughes
- Mary Todd Lincoln
- Graham Beckel
- Edwin Stanton
- Shawn Pyfrom
- Private John W. Nichols
- Greg Cooper
- Alexander Gardner
- Andrew Collie
- James McCollom
- Jeremy Ambler
- George Robinson
- Joe Inscoe
- Doctor May
- Benjamin Perkinson
- Tad Lincoln
- Logan Bennett
- John McCullough
- Geoffrey Culbertson
- Honor Guard Soldier
- Leiv Clegg
- Proud Slave
- Paul W. Carroll
- Admiral Porter
- Ken Peebles
- Old Barefoot Slave
- Michael Goodwin
- Captain Arvold
- Samuel Hunt
- W. H. Crook
- Lance Lemon
- William Bell
- David Furr
- Frederick Seward
- Ted Johnson
- William H. Seward
- Audra Honaker
- Fanny Seward
- Brett Dalton
- Robert Todd Lincoln
- Mike Marunde
- General Ulysses S. Grant
- Ryan G. Dunkin
- Harry Clay Ford
- Lynwood Bradley
- William H. Flood
- Mark Scarboro
- John Mathews
- Ford Flannagan
- E. A. Emerson
- Blythe Coons
- Laura Keene
- Paul D'Elia
- Charles Forbes
- Todd Fletcher
- Edman Spangler
- Joseph Carlson
- Major Henry Rathbone
- Eleanor Drew
- Clair Harris
- Matt Hackman
- Dr. Charles Leale
- Johnathan Tchaikovsky
- Lieutenant A.M.S. Crawford
- Levi Freeman
- William Kent
- Willie Repoley
- Dr. Charles Taft
- Shane Callahan
- Lieutenant J.T. Bolton
- Terry Jernigan
- Lieutenant Jameson
- Clayton Stocker Myers
- Henry B. Safford
- Keith Tyree
- General Christopher Augur
- Nick L. Williams
- Albert Daggett
- Kam Dabrowski
- Corporal James Tanner
- Seamus Mulcahy
- David Herold
- Richard Warner
- Dr. Ezra Abbott
- Myke Holmes
- Dr. F.A. King
- William L. Thomas
- Surgeon General Joseph Barnes
- Vince Nappo
- Harry Hawk
- Christopher Cartmill
- James Ferguson
- Josh Murray
- Lewis Powell
- Robert Neal Marshall
- Lawyer B.A. Hill
- Durrell Nelson
- Rev. Phineas Gurley
- Jaiden Kaine
- Oswell Swann
- Kevin Lawrence O'Donnell
- Samuel Cox
- Thomas K. Belgrey
- Colonel Lafayette Baker
- Lancer Dean Shull
- Lieutenant Edward Doherty
- Robert Wray
- Lieutenant Colonel
- Barry Ellenberger
- Lieutenant Luther Byron Baker
- Mark Halpern
- George Atzerodt
- Jim Wilson
- Barber
- Sara Eshleman
- Asia Booth Clarke
- Jen Downey
- Mary O'Melia
- Raynor Scheine
- Samuel Mudd
- Karla Brown
- Singing Woman
- Nicholas Angelo Batten
- The Light Guard
- Sarah Bella
- Miss Mary Meredith
- Zach Bowman
- Confederate Prisoner / Commoner
- John E. Daniel
- Telegraph operator
- Noah Downs
- Telegraph Operator
- Ronson Giggetts
- Contraband / Laborer (credit only)
- Lucas N. Hall
- Union Soldier
- David Leehy
- Rebel POW
- Gerald Poole
- Marine Guard
- Andrew Ragan
- Union Soldier
- George Reuther
- Theater Patron
- Laura Rikard
- Attractive Woman
- Dennis W. Sparks
- Congressman
- Robin Lord Taylor
- Sgt Silas Tower Cobb
- Joseph S. Timberlake
- Union Soldier
- Jim Batchelder
- Southern spy
- Chris Bookless
- Lieutenant W.W. Clemens
- Wendy Carter
- Mrs. Dixon
- Derek Chouinard
- Union Sailor
- Jeb Cooke
- Union Soldier
- Ed Easterling
- Chief Justice Cartter
- James 'Ike' Eichling
- Gidgeon Wells Sect Navy
- Vince Eisenson
- J.L. Debonay
- Brian Elder
- Sailor
- David Foster
- James Gifford
- Chance Gibson
- McGowan
- Jef Gums
- Emrick Hansell
- Steve Gums
- Augustus Seward
- Ramsey Hanchette
- The Naked Woman
- Jenny Howard
- Augusta Mountchessington
- Shaun Irving
- John F. Parker
- Raymond H. Johnson
- Ford's Theatre Patron
- Tamara Johnson
- Mrs. Mountchessington
- Christy Leigh
- The Prostitute
- George Ludden
- Richard Garrett
- Ed Mantell
- Disgruntled Richmond Gentleman
- Robert Lucas Nelson
- Richmond Man
- Roger Novak
- John Buckingham
- Dakota Oher
- Union Soldier
- Matthew Pabo
- Joseph 'Peanuts' Burroughs
- Mike Shiflett
- Hon. Hugh McCullough
- Paul Stober
- John Lloyd
- Eric Stoupa
- Congressman
- Amy Westmoreland
- Theater Patron
- Lawrence Whitener
- Lincoln's Photographer
- Mark E. Young Sr.
- Blacksmith and Union Soldier
