- Laura Bailey
- Black Widow / Little Girl / Sister (voice)
- Haley Joel Osment
- Taneleer Tivan / The Collector (voice)
- Will Friedle
- Hydra Goon 3
- James Mathis III
- Black Panther
- Liam O'Brien
- Johann Schmidt / Red Skull / Hydra Goon 2 (voice)
- Laura Post
- Betty Ross / Red She-Hulk / Milk Woman / Computer Voice (voice)
- Steve Blum
- Wolverine
- Trevor Devall
- Red Guardian
- Bumper Robinson
- Captain America / Sam Wilson (voice)
- Roger Craig Smith
- Captain America / Steve Rogers (voice)
- Fred Tatasciore
- Hulk
- Travis Willingham
- Thor Odinson
- Mick Wingert
- Iron Man / Tony Stark (voice)
