- James Brovington
- Â Peter Parker / ... 69 episodes, 2017-2020
- Emily Rose
- Â Gwen Stacy 40 episodes, 2019-2020
- Shawn Buffington
- Â J. Jonah Jameson / ... 38 episodes, 2017-2020
- Brendan Wreay
- Â Harry Osborn 25 episodes, 2018-2020
- Joy Pyper
- Â Aunt May 18 episodes, 2017-2020
- Meggie-Elise
- Â Gwen Stacy / ... 11 episodes, 2018-2019
- Jacob M. Keene
- Â Kingpin / ... 11 episodes, 2018-2020
- Thunder Muffin
- Â Hobgoblin / ... 8 episodes, 2018-2020
- Chris Wilson
- Â Miles Morales / ... 8 episodes, 2020
- Chrissy Pixy
- Â Mary Jane Watson 8 episodes, 2019-2020
- Christopher Zuckerman
- Â Captain George Stacy 7 episodes, 2018-2019
- Eman Sdrawkcab
- Â Flash Thompson / ... 7 episodes, 2018-2020
- Andrew Mondia
- Â Adrian Toomes / ... 7 episodes, 2017-2020
- Steve Stanley
- Â Roderick Kingsley / ... 7 episodes, 2018-2020
- Carrick Inabnett
- Â Electro / ... 7 episodes, 2018-2020
- Mackenzie Bryant
- Â Gwen Stacy / ... 6 episodes, 2017-2018
- Ryan Wichael
- Â Ned Leeds / ... 6 episodes, 2018-2019
- Erik Smith
- Â Mac Gargan / ... 6 episodes, 2019-2020
- Alexis Barnes
- Â Jean DeWolff / ... 5 episodes, 2018-2020
- Jorge Hernandez
- Â Daredevil / ... 4 episodes, 2018-2020
- Nathan Scars
- Â Doctor Bromwell 4 episodes, 2020
- ShadowGamingVA
- Â Betty Brant 4 episodes, 2019-2020
- Stan Lee
- Â Self / ... 3 episodes, 2017-2018
- Patrick Amgar
- Â Electro / ... 3 episodes, 2018-2020
- Kyle Souza
- Â Beetle / ... 3 episodes, 2019
- David Guerrero
- Â Hydro-Man / ... 3 episodes, 2019-2020
- Mike Cochran
- Â Bartender / ... 3 episodes, 2020
- Abigail Turner
- Â Mary Jane Watson 2 episodes, 2018
- Christian B.
- Â Frank Castle / ... 2 episodes, 2019-2020
- DoshVO
- Â Alistar Smythe / ... 2 episodes, 2019
- Asaf Arvizu
- Â Richard Parker 2 episodes, 2020
- Delaney Dgs
- Â Mary Parker 2 episodes, 2020
- Cap Cavern
- Â Miss Hudson 2 episodes, 2019
- Vincent Helices
- Â Jefferson Davis / ... 2 episodes, 2019-2020
- Da Start
- Â Kingpin Thug 2 episodes, 2020
- Terrence White
- Â Sin-Eater / ... 2 episodes, 2020
- Crab Tree
- Â Phil Urich 1 episode, 2019
- El Williams
- Â Ashley Kafka 1 episode, 2020
- Michael Bay
- Â Self 1 episode, 2018
- Fred Tatasciore
- Â Hulk 1 episode, 2018
- Siri
- Â Self 1 episode, 2018
- Tommy Wiseau
- Â Self 1 episode, 2020
- Samantha Moore
- Â Silver Sable 1 episode, 2019
- Georgia Elizabeth
- Â Anita Dillon / ... 1 episode, 2020
- Donald Misener
- Â Stan Carter 1 episode, 2019
- Jason Bawden
- Â Silvermane 1 episode, 2019
- Christine Marie
- Â Francine Lynn 1 episode, 2020
- Camden Boomershine
- Â Henry 1 episode, 2020
- West McDonald
- Â Wolverine 1 episode, 2020
- Katie Gallagher
- Â Green Goblin Hench-Woman 1 episode, 2020
- Luis Solana
- Â Cop 1 episode, 2019
- Matthew Judd
- Â Danny Dillon 1 episode, 2020
- Luis Carlos Ojeda
- Â Crusher Hogan 1 episode, 2017
