- Shôtarô Morikubo
- Koji Kabuto
- Ai Kayano
- Sayaka Yumi
- Sumire Uesaka
- Lisa
- Toshihiko Seki
- Tetsuya Tsurugi
- Ami Koshimizu
- Jun Hono
- Junpei Morita
- Prime Minister Yumi
- Unshô Ishizuka
- Dr. Hell
- Keiji Fujiwara
- Count Brocken
- Hiroyuki Miyasako
- Baron Ashura (male side)
- Romi Park
- Baron Ashura (female side)
- Natsuki Hanae
- Shiro Kabuto
- Wataru Takagi
- Boss
- Masami Kikuchi
- Nuke
- Kappa Yamaguchi
- Mucha
- Nana Yamaguchi
- Misato
- Bin Shimada
- Dr. Nossori
- Kôzô Shioya
- Dr. Sewashi
- Azusa Tadokoro
- Mazin Girl
- Miku Itou
- Mazin Girl
- Okarina
- Mazin Girl
- Yui P.
- Mazin Girl
- Sara Blum
- Additional Voices
- J. David Brimmer
- Boss
- Tyler Bunch
- Gennosuke Yumi
- James Weaver Clark
- Additional Voices
- Leigh Craig
- Misato
- AnnaBelle Deaner
- Mistato's Daughter
- Michael Deaner
- Child
- Daniel J. Edwards
- Conductor
- Matt Giroveanu
- Additional Voices
- Wayne Grayson
- Koji Kabuto
- Dan Green
- Tetsuya Tsurugi
- Jason Griffith
- Mucha
- Martha Harms
- Baron Ashura (Female) / Train Announcer (voice)
- Brian Kim McCormick
- Additional Voices
- Eddy Lee
- Shiro Kabuto
- Bill Lobley
- Count Brocken
- David Manis
- Dr. Sewashi
- Rob Morrison
- Fukurokoji
- Sarah Natochenny
- Additional Voices
- Jake Paque
- Yamagishi
- Ben Phillips
- Additional Voices
- Mike Pollock
- Dr. Hell
- Alyson Leigh Rosenfeld
- Sayaka Yumi
- Courtney Shaw
- Lisa
- Jason Simon
- Joint Force Command
- Michael Sinterniklaas
- Additional Voices
- Marc Swint
- Additional Voices
- Billy Bob Thompson
- Nuke
- Marc Thompson
- Baron Ashura (Male)
- Bill Timoney
- Dr. Nossori
- Kymberly Tuttle
- Jun Hono
