- John Cleese
- Llama lecturer / Second Yorkshireman / Armless Officer / Pope Julius II / Vocational Guidance Counsellor / Officer Praline / Self / Pepperpot / Second Penguin on Telly Pepperpot / Albatross Seller / Miss Anne Elk / Mr. Barnard / Eric Praline
- Terry Gilliam
- Spanish Dancer / Piano player / Pope's Servant / Mountie / Second Bruce / Constable Parrot / Self / Pepperpot / Professor D.P. Gumby / Sir Norman Barry Castle / Cardinal Fang / I've got two legs singer
- Eric Idle
- Spanish Guitarist / First Yorkshireman / Noël Coward / Michelangelo / First Bruce / Graham aka Hamrag Yatlerot / Self / First Poofy Judge / Arthur Nudge / Chris / Man in Pink / Mr. Bun
- Terry Jones
- Spanish Guitarist / Third Yorkshireman / Mrs. Blackitt / Mountie / Mr. Hilton / Self / Pepperpot / First Penguin on Telly Pepperpot / Albatross Person / Pub customer / Cardinal Biggles / Abuser / Spam Waitress
- Michael Palin
- Spanish Man / Fourth Yorkshireman / Harry Blackitt / Mr. Anchovy / Lumberjack / Third Bruce / Interviewer / Self / Pepperpot / TV Host / Second Poofy Judge / Nobby / Cardinal Ximenez / Argument Customer / Pet Shop Emplyee
- Graham Chapman
- The Colonel / Professor R.J. Canning / Batley Townswoman / Abe Seagull / Hegel / Tony Bennett (archive footage)
- Carol Cleveland
- Spanish Señorita / Virgin Mary / Best Girl / Punk Bruce / Mrs. Brown / Argument Secretary / Mrs. Bun / Self
- Brian Cox
- Self
- Samuel Holmes
- Various Roles
- Stephen Hawking
- Self
- Eddie Izzard
- Guest Bruce
- John Du Prez
- GMO Orchestra,piano
- Mike Myers
- Self
- Jeff Leach
- GMO Orchestra, keyboards
- Mike Davis
- GMO Orchestra, reeds
- Owain Harries
- GMO Orchestra, trumpet
- Nick Walsh
- GMO Orchestra, bass
- Steve Willingham
- GMO Orchestra, guitars
- Allan Cocks
- GMO Orchestra, drums
- Corrina Silvester
- GMO Orchestra, percussion
- Jamielisa Jacquemin
- Booth singer
- Sonia Jones
- Booth singer
- David Laudat
- Booth singer
- Melanie Marshall
- Booth singer
- Ashley Andrews
- Dance Ensemble
- Gabby Antrobus
- Dance Ensemble
- Curtis Angus
- Dance Ensemble
- Caoife Coleman
- Dance Ensemble
- Matthew Bradley
- Dance Ensemble
- Majella Fitzgerald
- Dance Ensemble
- Darron Gifty
- Dance Ensemble
- Lavinia Fitzpatrick
- Dance Ensemble
- Nick Gilligan
- Dance Ensemble
- Lauren Hearnden Mayer
- Dance Ensemble
- Jared Hageman
- Dance Ensemble
- Holly James
- Dance Ensemble
- Pip Hersee
- Dance Ensemble
- Hayley Martin
- Dance Ensemble
- Dominic Lamb
- Dance Ensemble
- Carly Meyers
- Dance Ensemble
- Kevin McGuire
- Dance Ensemble
- Joanna Rennie
- Dance Ensemble
- Matthew Wesley
- Dance Ensemble
- Lisa Spencer
- Dance Ensemble
- Ian Henderson
- MC
- Dominic Kinnaird
- Gilliam Cover
- Monty Python
- Themselves
- Warwick Davis
- Self
- Simon Jones
- Jeremy Portland-Smythe
- Dara Ó Briain
- Self
