- Jonathan Coy
- Leonato, Governor of Messina
- Leo Staar
- Messenger
- Catherine Tate
- Beatrice, Leonato's neice
- Sarah MacRae
- Hero, Leonato's daughter
- Adam James
- Don Pedro, Prince of Aragon
- David Tennant
- Benedick
- Elliot Levey
- Don John, bastard brother of Don Pedro
- Tom Bateman
- Claudio
- Anna Farnworth
- Innogen, Leonato's wife
- Lee Knight
- Conrade
- Alex Beckett
- Borachio
- Joshua Berg
- Titus, a servant in the house of Leonato
- Natalie Thomas
- Margaret, a maid in Leonato's house
- Kathryn Hunt
- Ursula, mother of Margaret
- Derek Howard
- Angelo, Ursula's husband
- Mario Marin-Borquez
- Boy, Maria's son
- Enzo Squillino Jr.
- Balthasar, a local musician / Sexton, a local official (as Enzo Squillino Jnr.)
- John Ramm
- Dogberry, master constable
- Mike Grady
- Verges, his deputy
- Clive Hayward
- Hugh Oatcake, a member of the watch / Friar Francis
- Nicholas Lumley
- George Seacoal, a member of the Watch
- Hannah Warren-Green
- Maria