- Sarah Natochenny
- Ash Ketchum
- Dan Green
- Mewtwo
- Bill Rogers
- Brock
- Michele Knotz
- Jessie / Misty / Bulbasaur / Togepi / Psyduck / Squirtle / Additional Voices (voice)
- Ikue Ôtani
- Pikachu
- Alyson Leigh Rosenfeld
- Nurse Joy
- James Carter Cathcart
- James / Meowth / Fergus / Additional Voices (voice)
- Ted Lewis
- Giovanni / Corey (voice)
- Lisa Ortiz
- Neesha / Miranda / Additional Voices (voice)
- Billy Bob Thompson
- Dr. Fuji / Additional Voices (voice)
- Aaron Phillips
- Raymond
- Emily Bauer
- Officer Jenny
- Eddy Lee
- Researcher #1
- Sam Haft
- Researcher #2 / Additional Voices (voice)
- Rodger Parsons
- Narrator
- Justin Anselmi
- Additional Voices
- Suzy Myers Jackson
- Additional Voices
- Marc Thompson
- Additional Voices
- Rikako Aikawa
- Zenigame / Koduck / Rokon (voice)
- Yôsuke Akimoto
- Doctor
- Peter Anderson
- Additional Voices
- Emma Bunton
- Additional Voices
- Tiana Camacho
- (voice)
- Cam Clarke
- (voice)
- Michaela Dean
- Additional Voices
- Doug Erholtz
- Additional Voices
- Paul Greenberg
- (voice)
- Megumi Hayashibara
- Musashi / Fushigidane (voice)
- Lin Hu
- Takeshi
- Masachika Ichimura
- Mewtwo
- Inuko Inuyama
- Nyarth
- Unshô Ishizuka
- Narrator
- Mayumi Izuka
- Kasumi
- Raymond Johnson
- Raymond
- Madigan Kacmar
- Additional Voices
- Satomi Kôrogi
- Togepi
- Brenna Larsen
- (voice)
- Rica Matsumoto
- Satoshi
- Shin'ichirô Miki
- Kojiro / Lizardon / Charizard (voice)
- Enn Reitel
- Additional Voices
- Rino Romano
- (voice)
- Aiko Satô
- Sweet
- Nick Shakoour
- Additional Voices
- Stephanie Sheh
- Additional Voices
- Ayako Shiraishi
- Joy
- Michael Sinterniklaas
- Additional Voices
- Wataru Takagi
- Umio
- Yûji Ueda
- Takeshi
- Kôichi Yamadera
- Mew
