- Jack Wild
- Jimmy
- Billie Hayes
- Witchiepoo
- Martha Raye
- Boss Witch
- Cass Elliot
- Witch Hazel
- Billy Barty
- Googy Gopher / Orville Pelican
- Jane Dulo
- Witch
- Jan Davis
- Witch Way
- Princess Livingston
- Miss Flick
- Sharon Baird
- Shirley Pufnstuf
- Joy Campbell
- Orson / Cling / Various characters
- Roberto Gamonet
- H.R. Pufnstuf
- Andy Ratoucheff
- Alarm Clock
- Angelo Rossitto
- Seymore Spider / Clang
- Felix Silla
- Polkadotted Horse
- Johnny Silver
- Dr. Blinky / Ludicrous Lion
- Walker Edmiston
- Dr. Blinky / Ludicrous Lion / Seymore Spider / Candle / Hippie Tree (voice)
- Joan Gerber
- Dowager Tree / Granddaughter Clock / Freddy the Flute (some scenes) (voice)
- Allan Melvin
- H. R. Pufnstuf / Henrich Rat / Living Island Boat / Orville Pelican / Polka-Dotted Horse / Stupid Bat / West Wind (voice) (as Al Melvin)
- Don Messick
- Freddy the Flute / Googy Gopher / Orson Vulture (voice)
- The Krofft Puppets
- Various Characters
