- Mark Ricci
- Peter Parker / Spider-Man
- Cheryl De Luca
- May Parker
- Carter Purtelle
- Flash Thompson
- Lisa Kovack
- Mrs. Winterhalter
- Albert Duic
- Captain Stacy
- Katerina Kova
- Ana Kravinoff (Remastered Edition)
- Brad Bryans
- Mr. Birkenstock
- Kaleb Carmichael
- Harry Osborn
- Ricar Giachini
- Kraven the Hunter
- Sydney Hall
- Mary Jane Watson
- Steve Kerr
- Wilson Allan
- Madi King
- Felicia Hardy
- Vijai Kumar
- News reporter #1
- Danny Loduca
- Hoffman
- Akins Lucas
- Dennis Carradine
- Yury Mitroshin
- Nikolai Kravinoff
- Kristen Patterson
- Liz Allan
- Jay-kub Pilkington
- Aleksei Sytsevich
- Philip Ramolo
- Daily Bugle Victim
- Joe Ricci
- J. Jonah Jameson
- Rebecca Tandon
- Reporter
- Jordan Tomasone
- Mr. Cobwell
- Joseph Viola
- McBride
- Curtis Steven Wetselaar
- Midtown High Victim
