- Sara Ballantine
- Mary Jane Watson
- Christopher Daniel Barnes
- Spider-Man / Peter Parker (voice) (archive sound)
- Earl Boen
- Beyonder
- Roscoe Lee Browne
- Kingpin / Wilson Fisk (voice) (archive sound)
- Joseph Campanella
- Lizard / Dr. Curt Connors (voice) (archive sound)
- Cam Clarke
- Mr. Fantastic / Reed Richards (voice) (archive sound)
- Quinton Flynn
- Human Torch / Johnny Storm (voice) (archive sound)
- J.D. Hall
- Blade
- Robert Hays
- Tony Stark / Iron Man (voice) (archive sound)
- David Hayter
- Captain America
- Gary Imhoff
- Harry Osborn
- Nick Jameson
- Michael Morbius
- Tom Kane
- Dr. Doom
- Brian Keith
- Uncle Ben Parker
- Joan Lee
- Madame Web
- Stan Lee
- Stan Lee
- Gail Matthius
- Invisible Woman / Sue Storm (voice) (archive sound)
- Iona Morris
- Storm / Ororo Munroe (voice) (archive sound)
- Nichelle Nichols
- Miriam
- Patrick Pinney
- The Thing / Benjamin J. Grimm (voice) (archive sound)
- Neil Ross
- Green Goblin
- Rodney Saulsberry
- Robbie Robertson
- Efrem Zimbalist Jr.
- Dr. Octopus / Dr. Otto Octavius (voice) (archive sound)
