- David Kaye
- Â Optimus Prime / ... 42 episodes, 2007-2009
- Jeff Bennett
- Â Prowl / ... 39 episodes, 2007-2009
- Bumper Robinson
- Â Bumblebee / ... 39 episodes, 2007-2009
- Tom Kenny
- Â Isaac Sumdac / ... 36 episodes, 2007-2009
- Bill Fagerbakke
- Â Bulkhead / ... 36 episodes, 2007-2009
- Corey Burton
- Â Ratchet / ... 35 episodes, 2007-2009
- Tara Strong
- Â Sari Sumdac / ... 34 episodes, 2007-2009
- Townsend Coleman
- Â Sentinel Prime / ... 13 episodes, 2007-2009
- Phil LaMarr
- Â Jazz / ... 12 episodes, 2008-2009
- Cree Summer
- Â Blackarachnia / ... 6 episodes, 2007-2009
- Alexander Polinsky
- Â Headmaster / ... 4 episodes, 2008-2009
- Susan Blu
- Â Arcee / ... 4 episodes, 2008-2009
- John Moschitta Jr.
- Â Blurr 4 episodes, 2008-2009
- Lance Henriksen
- Â Lockdown 3 episodes, 2008-2009
- Peter Stormare
- Â Meltdown / ... 3 episodes, 2008
- Kath Soucie
- Â Professor Princess / ... 2 episodes, 2008
- Fred Willard
- Â Swindle 2 episodes, 2008-2009
- 'Weird Al' Yankovic
- Â Wreck-Gar / ... 2 episodes, 2008-2009
- Brian Posehn
- Â Nanosec / ... 2 episodes, 2008
- Kevin Michael Richardson
- Â Omega Supreme 1 episode, 2008
- Judd Nelson
- Â Rodimus Prime 1 episode, 2009
- George Takei
- Â Yoketron 1 episode, 2009
- John Mariano
- Â Dirt Boss 1 episode, 2009