- Gregory Abbey
- Yusei Fudo
- Matthew Labyorteaux
- Jaden Yuki
- Dan Green
- Yugi Muto / Yami Yugi (voice)
- Sean Schemmel
- Paradox / Professor Viper (voice)
- Ted Lewis
- Jack Atlas / Chumley Huffington (voice) (as Ed Paul)
- Tom Wayland
- Crow Hogan
- Erica Schroeder
- Akiza Izinski / Dark Magician Girl (voice) (as Bella Hudson)
- Eileen Stevens
- Luna / Leo / Yubel (voice)
- Wayne Grayson
- Lyman Banner / Solomon Muto / Syrus Truesdale (voice)
- Darren Dunstan
- Maximillion Pegasus
- Yuya Miyashita
- Fudô Yûsei
- Kenichiro Ohashi
- Yûki Jûdai
- Shunsuke Kazama
- Yugi Muto / The Pharaoh (voice)
- Atsushi Tamura
- Paradox
- Takanori Hoshino
- Jakku Atorasu
- Shintarô Asanuma
- Kuro Hogan
- Ayumi Kinoshita
- Aki Izayoi
- Yuka Terazaki
- Luca
- Ai Horanai
- Lua
- Kappei Yamaguchi
- Daitokuji
- Hiromi Tsuru
- Yubel
- Tadashi Miyazawa
- Sugoroku Mutou
- Jiro Takasugi
- Pegasus J. Crawford
- Madeleine Blaustein
- Sartorius
- Marc Diraison
- Additional Voices
- Jason Griffith
- Atticus Rhodes
- Christie Moreau
- Alexis Rhodes
- Emlyn Elisabeth Morinelli
- (voice) (as Anna Morneau)
- Jonathan Todd Ross
- Yami Marik
- Tony Salerno
- Chazz Princeton
- Marc Schneider
- (voice)
- Rebecca Soler
- Additional Voices
- Eric Stuart
- Seto Kaiba / Bastion Misawa (voice) (archive footage)
- Murray Thom
- Rex Goodwin
- Marc Thompson
- Kagemaru (Young) / Roman Goodwin (voice) (archive footage)
