Yamato Kazakiri, a young zoologist, comes across a mysterious cube in the woods that transports him to a place inhabited by animal-headed humanoids known as the Zyumans.
Sela and Leo are kidnapped by the Deathgalien Player Amigarudo to be in his "Battle Show" where two people enter, and only one can leave, and he chooses best friends because it entertains him.
While chasing down the Deathgalien Player Gaburio, Yamato and the others find a gorilla Zyuman named Larry who has been studying humans for several years. Elsewhere, Azarudo lands on Earth to make Gaburio more powerful.
Kubaru appears and transforms Amu into one of his servants. To face this unexpected crisis, the Zyuohgers receive some unexpected help from Kamen Rider Ghost.
The Deathgalien Player Yabiker attacks with the maddening sound horn of his horn, posing as a challenge to the Zyuohgers, while Leo befriends a young musician after hearing one of his songs.
The Zyuohgers keep defeating the same enemy again and again, unaware that they are under the effect of the sleeping pollen of the Deathgalien Player Hanayaida.
Ginis, the leader of the Deathgaliens, envelops a large portion of the city into a barrier that disintegrates everything it touches, and the Zyuohgers run against time to find the switch that turns it down as it shrinks little by little, threatening everyone trapped inside, including themselves.
Despite escaping from the barrier, the Zyuohgers are easily defeated by Gift, the all-powerful destruction machine designed by Ginis. As Gift recharges to resume its attack, Sela and the other Zyumans confront the same "Bird Man" who once rescued Yamato and discover that the sixth Champion's Proof is in his possession. But when they realize that Yamato's true intentions were to send them home and confront Gift alone, the other Zyuohgers decide to return and help him instead, combining all their Zyuoh Cubes into a new, mighty robot.
After having a disastrous encounter with Genkuro Oiwa, a bookshop owner, Tusk is obliged to work for him during his recovery, while the other Zyuohgers are busy facing the Deathgalien Player Hattena who appears with a plan to destroy the culture of the Earth.
Amu befriends Kazuhiro Fuwa and his younger sister Marin, two siblings who are dealing with harsh times as Fuwa is working hard to make money for a surgery Marin needs in order to treat herself. However, upon knowing that he knows one of the Zyuohgers, the Deathgalien Player Dorobozu decides to exploit Fuwa's desperate need for money in order to lay a trap for Amu and the others.
The Deathgalien Player Hantajii shoots people left and right, transforming them into dolls with his sniper rifle and taking even the Zyuohgers by surprise. When only Leo and Tusk remain to face the enemy, they must find a way to outsmart the Deathgalien and save their friends.
Ginis's secretary Naria appears and starts looking for Zyumans by her master's orders, assisted by the Player Mantor, and when Yamato learns that his uncle Mario was kidnapped upon being mistaken by a Zyuman as well, he comes up with a risky plan to rescue him.
The Deathgalien Player Trumpas uses his cards to torment his victims and the Zyuohgers alike. Sela watches for a child who got lost from her father and manages to reunite them safely while bypassing the enemy's trick, but just when Trumpas is about to get his just desserts, the Zyuohgers are surprised with the arrival of "The World", a new and powerful enemy whose appearance and abilities are too familiar.
Trumpas reappears with a Blood Game even more sinister, but to stop him, the Zyuohgers must pass by The World, who easily defeated them in their last encounter, and proves himself a threat even more dangerous when he unveils his own set of Zyuoh Cubes.
Yamato discovers that The World is not working for the Deathgaliens out of his own will, but is under their control instead. The Zyuohgers then confront The World one more time, looking for a way to break him free.
Misao Mondo was released from the Deathgaliens' control, but he regrets all he had done as The World nonetheless and declines the Zyuohgers' offer to become their ally, until Yamato's companions are captured and he asks for Misao's help to rescue them.
While confronting the Deathgalien Player Prisonable, Tusk and Misao end up imprisoned inside an underground dungeon. With Misao blaming himself for putting themselves in such predicament, Tusk resigns himself to look for an exit alone, until Misao decides to rise up and come to his aid, assisted by a new ally that awakens just in time to help them.
When a bicycle explodes just after he touches it, Misao believes that he was afflicted with some kind of curse, until he learns that it was just a trick from another Deathgalien Player, who set up a massive bomb in the city, and the other Zyuohgers discover, much to their surprise, that Misao has a new, unexplored power that is the key to find it.
Bangray, the Megabeast Hunter, arrives on Earth looking for some new prey and gets himself involved in the Blood Game when he proves himself a powerful foe against the Zyuohgers, drawing the attention of the Deathgaliens.
After being convinced by Ginis to join the Deathgaliens, Bangray attacks Yamato and knocks him unconscious. Once awakening, he meets his mother, who was supposed to be dead, and by the time his friends discover the true nature of Bangray's powers, they rush to help him.
Worried that Yamato may be still afflicted by the events of the last battle, Misao comes across a way to cheer him up, unaware that he fell into a scheme from another Deathgalien Player. Meanwhile, Kubaru is in distress with the fact that Bangray have read his mind and attacks the hunter in order to silence him.
Yamato meets Daichi Hayashi, a friend from college and is invited to his marriage. With no leads regarding the prey he is looking for on Earth, Bangray decides to torment Yamato once more by attacking the ceremony, and the other Zyuohgers decide to fight the intruder by themselves.
In another plot to make the Zyuohgers suffer, Bangray makes copies of Sela and Tusk and rigs them along the original ones with explosives, giving Yamato and the others 30 minutes to figure out the impostors and slay them before the bombs detonate, killing all four of them.
Captain Marvelous from the Gokaigers appear and reclaims a mysterious artifact that was hidden inside the Link Cube, but the Zyuohgers refuse to let him take a relic from Zyuland and fight him for its possession. However, Bangray appears to steal it as well, and a three-way battle begins.
The Zyuohgers and the Gokaigers join forces against Bangray, who summons fallen enemies of the previous Super Sentai to fight by his side. In the occasion, Yamato unlocks the powers of the Great Champion's Proof and gains a new transformation.
Having obtained the Whale Change Gun, the Zyuohgers begin their search for the ultimate Zyuoh Cube, Cube Whale. However, Bangray stands on their way once again, as the Megabeast Hunter intends to make Cube Whale his 100th prey.
The Zyuohgers are having a hard time trying to calm down Cube Whale, while fighting to protect it from Bangray and the Deathgaliens, but to make matters even worse, Ginis sends an upgraded version of his Gift destruction machine upon them.
The Blood Games start again and the Zyuohgers come across an enemy that brings forth their hidden, darkest feelings. Misao, the only one unaffected by its power, becomes afraid of his friends' new attitude, until he decides to stop running away and confront them.
Bangray kidnaps Yamato and demands the other Zyuohgers to hand over Cube Whale as ransom. To face their biggest crisis so far, the Zyuohgers assemble all their Zyuoh Cubes into their most powerful mecha ever.
Halloween has come and while fighting to protect a girl called Mami from the Deathgaliens, Tusk earns her affection, to the point that she starts viewing him as her enchanted prince, much to his chagrin.
The Zyuohgers discover a giant Zyuoh Cube and while trying to activate it they are drawn into another of the Deathgaliens' Blood Games, until Bud, the Bird Man reappears, using the Champion's Proof in his possession to transform into a Zyuohger as well.
Having transformed into Zyuoh Bird, Bud helps the other Zyuohgers against the Saguil Brothers, and reveals to Yamato the reason why he stole the Champion's Proof, claiming that Zyuland must be separated from the human world no matter the cost.
Deciding to personally involve himself in the Blood Game, Ginis captures Misao with the Zyuohgers confronting the alien to rescue their friend. Quval attempts to take advantage of the situation to have his revenge on Ginis, only for the Deathgalien leader to reveal his true power.
The Zyuohgers take the injured Misao to the care of a doctor, who coincidentally, is Yamato's father and return to get back at Quval for kidnapping their friend. Still terrified upon seeing the true power of Ginis, Quval makes one desperate last stand against them.
Christmas is at hand and the Zyuohgers join the festivities. When the Deathgaliens attack them, Mario witnesses their battle, and accidentally discovers Yamato's secret.
Having discovered their secret, Mario hears all the truth from Yamato and the others. Meanwhile, Bud meets Larry and the two try to analyze together the piece left from Azald.
While fighting the Zyuohgers, Azald reverts to his original form, and he is revealed to be the same monster that once attacked Earth and was defeated by Cetus, the original Zyuoh Whale.
With both team leaders gone, Ginis decides to personally end the 100th Blood Game by infusing some his own cells onto the Earth, causing it to start cracking. Though Bud comes with a plan to save the planet, it would permanently severe Earth's connection to Zyuland.
